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| - [21:55:25] IMAGE: [21:56:32] IMAGE: [21:57:14] IMAGE: [21:57:36] IMAGE: [21:57:58] and here [21:58:01] IMAGE: [21:58:21] so end up with 3 sheet groups [21:58:23] IMAGE: [21:58:55] couple bands to hold together [21:58:58] IMAGE: [21:59:15] a couple to pull it together [21:59:17] IMAGE: [21:59:34] IMAGE: [22:00:07] now is where i would normally look at server prediction on puzzle page [22:00:54] there is enough chance that the 3 helix segments are one helix (since it's not likeley there at all anyway, to join them into one [22:01:04] IMAGE: [22:01:54] rebuild into helix shape [22:02:12] IMAGE: [22:02:47] freeze structures [22:02:51] IMAGE: [22:03:45] start with first sheet group [22:03:53] IMAGE: [22:04:08] going to shift middle sheet to put phobics on same side as other sheet [22:04:19] IMAGE: [22:04:38] band [22:04:41] IMAGE: [22:05:18] wiggle them together [22:05:31] IMAGE: [22:05:46] i think that right one will come down a couple segments, but we will see [22:06:18] band next set and wiggle together [22:06:22] IMAGE: [22:06:55] helix in the way so band it out and rebuild to move [22:06:58] IMAGE: [22:07:04] IMAGE: [22:07:27] tweak shift sheet [22:07:34] IMAGE: [22:07:54] reband [22:08:04] and wiggle [22:08:07] IMAGE: [22:09:18] won't pull together so rebuild turn [22:09:21] IMAGE: [22:09:50] STILL WON'T PULL TOGETHER SO SHORTEN SHEET [22:10:41] IMAGE: [22:11:04] join 2 short sheets [22:11:07] IMAGE: [22:12:48] IMAGE: [22:13:42] sometimes you have to move the larger sidechains to get the sheets to pull together or the sidechains will push them away from each other, or make only part connect [22:15:30] IMAGE: [22:15:38] had to kill the two single helix dots at the ends of the sheets to get them to connect too [22:16:09] now shake and wiggle out [22:18:13] dosome inor rebuilds to clean up turns between sheets to free up where they are binding and not connecting with h bonds [22:18:24] IMAGE: [22:19:21] and it's still rough but coming together [22:19:36] shake and wiggle out to get sheets to settle in together [22:19:42] IMAGE: [22:20:41] now to try and figure out hwo the three sets of sheets come together and where to stick the helix [22:21:10] The place where I usually get stuck. [22:21:21] a lot of large sidechains on this one, tryptophan, tyrosine, and phenalalanine [22:21:50] those often group together and stack on top of each other, not so much trpy but the other two [22:22:40] so i'm going to take a stab at it that they gome somewhere in these areas and band [22:22:45] IMAGE: [22:23:29] IMAGE: [22:23:34] do you change options on those rubber bands? or just go with the default? [22:23:40] default [22:24:00] can weaken thme if you want, but i usually just wiggle in short bursts to keep it all together [22:24:24] IMAGE: [22:24:33] one set is trying to pull to the side of the other [22:25:06] i'l take that as a sign from the folding gods [22:25:09] IMAGE: [22:25:51] you just became my handfold god [22:25:57] lol [22:26:01] IMAGE: [22:26:03] heh [22:26:07] other one wants in the act too [22:26:13] IMAGE: [22:26:56] it's ugly with mismatched sheets [22:27:02] different lengths and all together [22:27:26] IMAGE: [22:27:50] bring helix in [22:27:53] IMAGE: [22:29:29] and now try to figure out a way to make it look liek a real protein [22:29:36] unfreeze [22:29:40] IMAGE: [22:29:44] shake wiggle [22:30:21] sometimes I wish I could physically take them in my hands and shape them... [22:30:34] maybe one day haptics will be good enough... [22:30:38] there are ways [22:30:40] lol [22:31:25] after its settled together ok, kill bands [22:31:29] IMAGE: [22:31:41] wiggle it out [22:32:19] IMAGE: [22:32:38] ;) fin ! I think [22:32:49] now comes the hard part of trying different configurations [22:33:32] i will usually rebuild out the reddest spots first though to get rid of kinks and have an idea of where different sheet placements will score [22:40:05] <@marie_s> for precision , al was ci=1? [22:40:12] YEAH [22:40:22] I DON'T BOTHER WITH CI TILL LATER USUALLY [22:40:27] stupid cpas key [22:40:46] beset by typo demons [22:41:11] i think i dropped it to about .15 once to shake when a lot of stuff was bunched together
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![[RDF Data]](/fct/images/sw-rdf-blue.png)