The Temple Sanctuary is a room in the Great Temple. This is the room where Samus faces several Splinters and an Alpha Splinter before they eventually turned into Dark Splinters and a Dark Alpha Splinter. She gains the Energy Transfer Module following their defeat. This is the third room in the Great Temple. This room had many gates guarding the paths to Agon, Torvus and Sanctuary Sectors. The Dark Aetherian version of this room is the Sanctum. This room also contains the sleeping Luminoth in cryo-stasis; they are the last members of their species.
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