Twilight Sparkle's human counterpart appears and is mentioned throughout the My Little Pony Equestria Girls film series. She is a student at Canterlot High School, a former student of Crystal Prep Academy, and the owner of Spike's counterpart, Spike the Dog. This version of Twilight looks considerably different from her pony counterpart as she wears thick black glasses and her hair in a bun. She is named Sci-Twi in some merchandise and other depictions.She appears in My Little Pony Equestria Girls: Friendship Games as the deuteragonist, but later turned into her alter ego, Midnight Sparkle, who briefly serves as the climax antagonist in My Little Pony Equestria Girls: Friendship Games.
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