The N terminal of a protein is the first segment (or residue) in the protein. The "N" refers to the nitrogen atom in the "amine group" of an amino acid. Proteins are built in a specific order. The N terminal residue is always one that was added before any others. Methionine is always the first amino acid added to a protein, but it's often removed by a post-translational modification (PTM). The order of segments is important. The last segment of a protein is called the C terminal.
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