According to Edward Spiegel, the soap concentration of bubble juice left in the wand increases as bubbles are made. This phenomenon is especially noticeable with tri-string wands when creating many bubbles from a single dip or when trying to make a second bubble after making a long tube. As multiple bubbles are made from the same dip, a distinct shift in color profile (which indicates film thickness and detergent concentration) will be seen. This is also noticeable when making very long tubes under good conditions (so that changes in color are not due to evaporative thinning). This is especially noticeable when using mixes at or above the Critical Micelle Concentration. The last bubbles will often be transparent or golden.
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