About: Microtome, Cryostat   Sponge Permalink

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A cryotat microtome (from the Greek mikros, meaning "small", and temnein, meaning "to cut") is a sectioning instrument that allows for the cutting of frozen samples, many rotary microtomes can be adapted to cut in a liquid nitrogen chamber, in a so-called cryomicrotome setup. The reduced temperature allows for the hardness of the sample to be increased, such as by undergoing a glass transition, which allows for the preparation of semi-thin samples. However the sample temperature and the knife temperature must be controlled in order to optimise the resultant sample thickness.

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  • Microtome, Cryostat
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  • A cryotat microtome (from the Greek mikros, meaning "small", and temnein, meaning "to cut") is a sectioning instrument that allows for the cutting of frozen samples, many rotary microtomes can be adapted to cut in a liquid nitrogen chamber, in a so-called cryomicrotome setup. The reduced temperature allows for the hardness of the sample to be increased, such as by undergoing a glass transition, which allows for the preparation of semi-thin samples. However the sample temperature and the knife temperature must be controlled in order to optimise the resultant sample thickness.
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abstract
  • A cryotat microtome (from the Greek mikros, meaning "small", and temnein, meaning "to cut") is a sectioning instrument that allows for the cutting of frozen samples, many rotary microtomes can be adapted to cut in a liquid nitrogen chamber, in a so-called cryomicrotome setup. The reduced temperature allows for the hardness of the sample to be increased, such as by undergoing a glass transition, which allows for the preparation of semi-thin samples. However the sample temperature and the knife temperature must be controlled in order to optimise the resultant sample thickness.
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