| abstract
| - Puzzle 400 is at:
*
* There was neither <150 nor <15 versions of this puzzle The puzzle started on 02/15/2011 and ended on 02/23/2011 (extended 1 day due to server downtime) It was a de-novo puzzle. The top score was 12,002 (solo score by aap). Several people kindly posted screenshots of their top solutions: Evolver solutions:
* Darknight900's 1st place solution: irc_200415_1298502265.png|DK 400 Solution|link=
* auntdeen's 2nd place solution:
* Hanto's 3rd place solution:
* ziiONIC's 4th place solution:
* rav's 5th place solution: Solo solutions:
* aap's 1st place solution: irc_101291_1298502155.png|aap 400 Solution (1 of 3)|link= irc_101291_1298502184.png|aap 400 Solution (2 of 3)|link= irc_101291_1298502222.png|aap 400 Solution (3 of 3)|link=
* Mark-'s 2nd place solution: irc_115025_1298502266.png|Mark- 400 Solution (1 of 3)|link= irc_115025_1298502422.png|Mark- 400 Solution (2 of 3)|link= irc_115025_1298502248.png|Mark- 400 Solution (3 of 3)|link=
* keypad5's 3rd place solution: irc_122900_1298502352.png|keypad5 400 solution (1 of 3)|link= irc_122900_1298502398.png|keypad5 400 solution (2 of 3)|link= irc_122900_1298502470.png|keypad5 400 solution (3 of 3)|link=
* CFC's 4th place solution: irc_102459_1298502414.png|CFC's 400 solution|link=
* grabhorn's 5th place solution: irc_179021_1298500558.png|Grabhorn's 400 solution (1 of 4)|link= irc_179021_1298502397.png|Grabhorn's 400 solution (2 of 4)|link= irc_179021_1298502409.png|Grabhorn's 400 solution (3 of 4)|link= irc_179021_1298502420.png|Grabhorn's 400 solution (4 of 4)|link=
* groover's 6th place solution: irc_224854_1298499753.png|Groover's 400 solution|link= Discussion: beta mentioned "Please try to use these secondary structure predictions: Here is the sequence logo predicted by the SAM server. H = helixE = sheetC = loop (or coil) The taller the letter at each position, the higher the probability of that specific secondary structure for that amino acid. For example, the amino acid Leucine at residue 26 is highly predicted to be part of a helix. However, the Leucine at residue 34 is predicted to have an equal probability of being anything." 2 recipes for setting the secondary structure were posted Mikedressner indicated "[...]The protein is Bile acid 7a-dehydratase It belongs to the bacteria organism Eubacterium sp. Catalyzes the dehydratation of 7-alpha,12-alpha-dihydroxy-3-oxochol-4-enoate. The enzyme can also use 7-alpha-hydroxy-3-oxochol-4-enoate as a substrate but not 7-alpha,12-alpha-dihydroxy-3-oxochol-5-beta-anoate, 3-alpha,7-alpha,12-alpha-trihydroxychol-5-beta-anoate or 7-beta-hydroxy-3-oxochol-4-enoate For more info: Go to the uniprot ascession number P19412"
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