An autoclave is a device to sterilize equipment and supplies by subjecting them to high pressure steam at 121° C or more. Sterilization (or sterilization, see spelling differences) refers to any process that effectively kills or eliminates transmissible agents (such as fungi, bacteria, viruses, spore forms, etc.) from a surface, equipment, article of food or medication, or biological culture medium. A widely-used method for heat sterilization is the autoclave, sometimes called a converter. Autoclaves commonly use steam heated to 121°C (250°F) or 134°C (270°F). To achieve sterility, a holding time of at least 15 minutes at 121°C or 3 minutes at 134°C is required. Additional sterilizing time is usually required for liquids and instruments packed in layers of cloth, as they may take longer to
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| - An autoclave is a device to sterilize equipment and supplies by subjecting them to high pressure steam at 121° C or more. Sterilization (or sterilization, see spelling differences) refers to any process that effectively kills or eliminates transmissible agents (such as fungi, bacteria, viruses, spore forms, etc.) from a surface, equipment, article of food or medication, or biological culture medium. A widely-used method for heat sterilization is the autoclave, sometimes called a converter. Autoclaves commonly use steam heated to 121°C (250°F) or 134°C (270°F). To achieve sterility, a holding time of at least 15 minutes at 121°C or 3 minutes at 134°C is required. Additional sterilizing time is usually required for liquids and instruments packed in layers of cloth, as they may take longer to
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| - An autoclave is a device to sterilize equipment and supplies by subjecting them to high pressure steam at 121° C or more. Sterilization (or sterilization, see spelling differences) refers to any process that effectively kills or eliminates transmissible agents (such as fungi, bacteria, viruses, spore forms, etc.) from a surface, equipment, article of food or medication, or biological culture medium. A widely-used method for heat sterilization is the autoclave, sometimes called a converter. Autoclaves commonly use steam heated to 121°C (250°F) or 134°C (270°F). To achieve sterility, a holding time of at least 15 minutes at 121°C or 3 minutes at 134°C is required. Additional sterilizing time is usually required for liquids and instruments packed in layers of cloth, as they may take longer to reach the required temperature (unnecessary in machines that grind the contents prior to sterilization). Following sterilization, liquids in a pressurized autoclave must be cooled slowly to avoid boiling over when the pressure is released. Modern converters operate around this problem by gradually depressing the sterilization chamber and allowing liquids to evaporate under a negative pressure, while cooling the contents.
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