Pioneer Bus Company was a bus operator in the borough of Brooklyn, New York City up to October 21, 1979. It was originally organized as a school bus operator in 1955, but in 1960 began operating local bus route B100 from Mill Basin to the Kings Highway subway station on the BMT Brighton Line in Brooklyn. In 1972, express bus service from Brooklyn to Manhattan was added to Pioneer's single local route. On October 22, 1979, its routes were taken over by Command Bus Company.