The first known Licker was a cluster developed within The Hive, one of the Umbrella Corporation's bio-weapons laboratories. One Licker was released from confinement to hunt down Umbrella's "Sanitation" taskforce by the Red Queen to prevent an outbreak of the virus spreading beyond the laboratory itself. The Licker killed Spence at the train platform, and after feeding on his body, mutated into a new form. The Advanced Licker then proceeded to follow Rain; Alice; Matt Addison and Chad Kaplan. The creature threw Kaplan straight out of the train, likely killing him, and scratched Addison, infecting him with a mutant strain of T-virus. Matt and Alice killed the Licker as they escaped from the train.