Throughout "I, Monster", various flashbacks reveal the origin of this series' version of the Rat King; the episode reveals the Rat King was originally the Slayer, from the episode "Bishop's Gambit", a prototype bio-mechanical super soldier created by Agent Bishop using genetically manipulated, alien, the turtles, his own and Splinter's DNA. The Slayer was meant to be used to fight off any alien threats and was given a test run against the Turtles, who broke into Bishop's laboratory looking for the kidnapped Splinter. The Slayer was presumed destroyed when a fight between it, the Turtles, Splinter and Bishop flooded the agent's lab. In actuality, the Slayer survived and was sent hurtling through the sewers, eventually being deposited near the industrial park the events of "I, Monster" take