The 140th New York Volunteer Infantry Regiment was a United States Federal military Regiment that was mustered on September 13, 1862 and was mustered out on June 3, 1865 for service in the American Civil War. Citizens of Monroe County, New York made up the majority of its members. Companies B, C, E, F, K and part of G were men from Rochester; the remaining four and a half companies were populated by citizens of the neighboring towns and villages.