27th U-boat Flotilla ("27. Unterseebootsflottille") was a training flotilla ("Ausbildungsflottille") of the German Kriegsmarine during World War II. The flotilla was founded at Gotenhafen in January 1940 under the command of Korvettenkapitän Ernst Sobe as Taktische Unterseebootsausbildungsflottille ("Tactical U-boat Training Flotilla"), and was redesignated 27. Unterseebootsflottille in June 1940.