During the HYDRA Uprising, S.H.I.E.L.D. suffered heavy casualties at the hands of HYDRA. Nick Fury gave Agent Bobbi Morse a secret misson to start Protocol ASTA06 aboard the aircraft carrier Iliad. During this battle on the Iliad, Morse found a team of S.H.I.E.L.D. agents, including Alphonso Mackenzie, who had been captured by HYDRA. Morse distracted them by throwing her phone at them before shooting most of them; she was assisted by Isabelle Hartley, who threw a knife at the Lead HYDRA Agent.