Ebenezer Sproat (1752–1805), surname also spelled Sprout, was an officer of the Continental Army during the American Revolutionary War, a pioneer to the Ohio Country, and one of the founders of Marietta, Ohio, the first permanent American settlement in the Northwest Territory. He served throughout the entire American war of independence, from April 1775 through November 1783, achieving the rank of colonel. After the war, he was a pioneer and surveyor in the Northwest Territory, and became a leader of the militia at Marietta during the Northwest Indian War. He was the first sheriff in the Northwest Territory and Ohio, serving fourteen years as sheriff of Washington County, the oldest county in Ohio.