The 16th Virginia Volunteer Infantry Regiment was an infantry regiment raised in Virginia for service in the Confederate States Army during the American Civil War. It fought mostly with the Army of Northern Virginia. The 16th Virginia completed its organization in May, 1861, with ten companies. However, because of various reorganizations and transfers, the unit contained only seven after November 1, 1862. The men were from Suffolk and Portsmouth and the counties of Nansemond, Isle of Wight, Sussex, and Chesterfield.