Because of the purity of their blood, and their unique nature, pure bloods are essentially the rulers of the vampire Houses and its major power-players, while non-pure bloods are essentially the lackeys, minions, and/or lieutenants within their respective Houses. Due to the gap in genetic and blood purity, pure bloods tend to look down on the non-pure bloods and the non-pure bloods look at the pure bloods with equal disdain, although many of the "turned" see the purebloods as superior, viewing them with such reverence, as to even consider them to be the gods of their world.
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