I'm not sure I agree with the concept of stiffening. So I'll throw out some thoughts and let others comment. I don't think wiggling "stiffens" a fold. If it did, then wiggling a denovo would make it into a stick you could not bend. Rebuilding seems to happen pretty easily, pulling segments seems to be pretty easy also. As an evolver I like to first settle a shared protein to evolve it. Then I know, I will get some credit for my effort. After it is evolved then, I try the longer running overnight scripts. I don't like to run a script all night just find it did not evolve at all.