Ferb Fletcher (Phineas and Ferb) as Batman/Bruce Wayne Phineas Flynn (Phineas and Ferb) as Robin/ Dick Grayson Dr. Doofenshmirtz (Phineas and Ferb) as The Joker The Coachman (Pinocchio) as The Penguin Candace Flynn (Phineas and Ferb) as Harley Quinn Vanessa Doofenshmirtz (Phineas and Ferb) as Catwoman Captain Hook (Peter Pan) as Two-Face Plankton ( SpongeBob Squarepants) as Mr. Freeze The Summerween Trickster (Gravity Falls) as Scarecrow Fire Lord Ozai (Avatar: The Last Airbender) as Ra's Al Ghul Frollo (The Hunchback of Notre Dame) as Hugo Strange