The cover features Samus Aran against a background similar to the game's Samus Data Screen. A pull-out poster is included with the guide. The "Official Perfect Guide" part of the guide's name is styled in the same font and style as the game's logo, against the Screw Attack symbol. The guide comes with a pull-out poster and divides the story into five chapters. Before this, there is a Basic Training section which goes over the controls for the game and the items, as well as certain techniques. There are also appendices detailing the locations of Missile Expansions, Energy Tanks, "secrets", Image Gallery and Maps, and a full walkthrough for the original Metroid, unlockable in Prime through the Nintendo GameCube Game Boy Advance Cable.