The Glycohemoglobin analyzer is a laboratory instrument that dilutes the whole blood specimen with Hemolysis & Wash Solution, and then injects a small volume of this specimen onto a Variant Column. Separation is achieved by utilizing differences in ionic interactions between the cation exchange group on the column resin surface and the hemoglobin components. The hemoglobin fractions (designated as A1a, A1b, F, LA1c+, SA1c, A0, and H-V0, H-V1, H-V2) are subsequently removed from the column by performing a step-wise elution using the varied salt concentrations in the Elution Buffers HSi Variant 1, 2, and 3.