Anacrothe was referenced in background to Nosgoth via the red sisters of Anacrothe/Alchemists class. Here he was initially cited along with the other members of the Dark Eden triad as an example of the elemental knowledge that was lost and became legend to the Humans under the rule of Kain's empire in the Soul Reaver era. After Elustra's destruction of her vampire alchemist masters and subsequent return of the knowledge to humans, she named her order the "red sistes of Anacothe", honoring the last human known to have possessed knowledge of alchemy.