Queen Penelobee is similar to Penelope in most aspects, but is unfamiliar with Betty (ironic as Betty and Penelope interact regularly) and has romantic feelings for Sparky, after he had posed as a "king bee" in "Bee Movie". It is only in "Queen For a Day" that Queen Penelobee and her "mother" Penelope meet in person (though Penelope believes the bees to be part of a virtual reality game); the bees of Droneopolis were willing to obey both due to their common hatred of Betty.