German submarine U-541 was a Type IXC U-boat of the Nazi German Kriegsmarine during World War II. She was laid down at the Deutsche Werft (yard) in Hamburg as 'werk' 362 on 5 May 1942, launched on 5 January 1943 and commissioned on 24 March with Kapitänleutnant Kurt Petersen in command. U-541 began her service career with training as part of the 4th U-boat Flotilla from 24 March 1943. She was re-assigned to the 10th flotilla for operations on 1 November, then the 33rd flotilla on 1 November 1944. She carried out four patrols and sank one ship. She was a member of four wolfpacks.