The 27th Virginia Volunteer Infantry Regiment was an infantry regiment raised in Virginia for service in the Confederate States Army during the American Civil War. It fought mostly with the Stonewall Brigade of the Army of Northern Virginia. The 27th fought at First Manassas, where it earned the nickname “the Bloody 27th” because of its losses, First Kernstown, and in Jackson's Valley Campaign. It then participated in the campaign of the Army of Northern Virginia from the Seven Days' Battles to Cold Harbor, moved with Early to the Shenandoah Valley, and was active around Appomattox.