The experiment was conducted in early 1944 by a Nazi scientist. However, before the serum could be tested, Spy Smasher took it and inadvertently killed the Nazi scientist who created it. He delivered the serum to his commanders at the O.S.S. The serum eventually found its way into a Cadmus storage facility. Cadmus scientists had tested the formula on lab animals, but it was unstable and caused disfigurement. General Wade Eiling took the formula to fight the Justice League after Cadmus stopped its operations.