There are only three direct allusions to the Guf within the show itself. In Episode 21' (during the Katsuragi Expedition footage): Woman D: "Begin thermal dampening treatment as soon as the Doors of Guf open." In Episode 23 (to Misato and Shinji in the Dummy Plug Plant): In Episode 26' (during Third Impact): Fuyutsuki: "The Chamber of Guf has been unsealed. Have the doors to the world's beginning and end finally opened?" In addition, the vulva-like apertures that open on Lilith's palms in 26' to receive souls are referred to as "Doors of Guf" in the relevant storyboard.