Dr. Pamela Isley began to create Animal-Plant Hybrids in a South American jungle laboratory that was funded by Wayne Enterprises. Her success in trying to complete and perfect the hybrid was interrupted constantly by the ambitious, but treacherous, Dr. Jason Woodrue. The toxic chemical, labelled "Venom" by those two doctors, was stolen from Isley's lab and was used in Woodrue's experiments to create the chemically altered human being known as "Bane". After she discovered Woodrue's experiment during an auction to world terrorist leaders, Isley refused to join Woodrue in his financial endeavors. Woodrue attempted to dispose of Isley by pushing her into a table of various poisons, toxins, and chemicals, but instead, caused her transformation into "Poison Ivy".