Born the son of an army surgeon in Darmstadt, Duesterberg entered the Prussian Army in 1893 after training in the cadet corps. In 1900, Duesterberg was part of the East Asian Expedition Corps that saw action in China during the Boxer Rebellion. Two years later, Duesterberg became an officer and held a variety of army commands prior to World War I. During the war, Duesterberg served in the Prussian War Ministry and eventually attained the rank of lieutenant colonel. Following the war, Duesterberg resigned from the army in protest over the Versailles Treaty, which Duesterberg viewed as being extremely unfair to Germany. Duesterberg subsequently decided to enter politics and joined the German National People's Party (DNVP) in 1919.