The Alley Cats are first seen after Puggsy and Frankie DaFlea are been captured by the Straycatchers led by Dr. Applecheeks, confronting Tom before they sing the "What do we care?" song. After finishing singing Tom sneaks away and one of them notices him escaping and they give chase knocking the flower pot down where Jerry is being held earlier by Tom, who then makes a run to a nearby manhole. As Tom passes the manhole, Jerry opens it and all of the Alley Cats fall into the sewer one by one saving Tom from further beating. The Alley Cats are not seen afterwards and it is unknown what happened to them, presumed they gave up after this.