Though mentioned during the Laboratory 5 arc, Laboratory 3 is first seen during the "Fishing" Mission orchestrated by Colonel Roy Mustang. After the body of Barry the Chopper is chased away from Warrant Officer Vato Falman's safehouse, Barry (in his armor vessel), Mustang, Lt. Riza Hawkeye, 2nd Lt. Jean Havoc and Alphonse Elric follow it into the depths of Lab 3, where the team splits off in different directions to find clues. While Barry, Hawkeye and Alphonse make it to the very doors of Father's lair, Mustang and Havoc encounter Lust in the human experimentation lab, to devastating results.