All the main characters from the previous three series appear with the exception of Smartotchi (although he appears in a flashback). There are also three new central characters first debuted in this series, Neenetchi, Orenetchi and Lovesoratchi. In addition, Oyajitchi appeared in the series as a supporting character, making GO-GO Tamagotchi! his first Tamagotchi! anime appearance. The season one opening theme is a new version of the classic opening theme, and the ending theme is a new song, entitled "RAINBOW". Both songs are performed by a Japanese idol singer named "hitomi".