Sam first meets Jo when he and Dean investigate a message on the dad's phone left by a women named Ellen. After they break into the Roadhouse, Ellen and Jo hold them at gunpoint until Ellen figures out their John's sons. They talk and find out Ellen and Jo know about the demon and knew John. At the end of the episode, Jo makes hints for Sam to go so that she can talk to his brother, Dean. The next time Sam and Jo meet, Sam comes into the Roadhouse and asks where Ash is, and she tells him. He then goes to talk to Ash without even saying hi to Jo.