A total of 16 clubs played in the fifth round, all winners of the fourth round. The draw was held on 30 January 2017, and the matches were played across the weekend of 18–20 February 2017. The round included the two teams from Level 5 that were still in the competition: Lincoln City and Sutton United, whose fourth round victories ensured the first ever fifth round with two non-league sides remaining. Lincoln City were in this round for the first time in 115 years and Sutton United for the first time in their history.