German submarine U-464 was a Type XIV supply and replenishment U-boat ("Milchkuh") of the Nazi German Kriegsmarine during World War II. Her keel was laid down on 18 March 1941, by Deutsche Werke in Kiel as 'werk' 295. She was launched on 20 December 1941 and commissioned on 30 April 1942 with Kapitänleutnant Otto Harms in command. He remained in charge for her entire career. The boat began her service life training in the 4th U-boat Flotilla before moving on to the 10th flotilla for operations.