Daisy was born in New York City, New York, the daughter of actress Sasha von Sherler and Paul Avila Mayer, and granddaughter of screenwriter Edwin Justus Mayer. After contributing to the New York Shakespeare Festival as a teen, Daisy graduated from Wesleyan University with a dregree in theater and history. Daisy's experience with theater served as a foundation for her upcoming career as a director, where she would later apply her understanding of stage acting to her work for the screen. Upon graduating interpretations of classic plays such as Euripides' Electra, Shakespeare's The Tempest, and Shakespeare's Two Gentlemen of Verona.